Recombinant Danio rerio Beta-actin/ACTB protein (His Tag)

Specifications

Catalog number

PDEO100020-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells. Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction. In addition to their role in the cytoplasmic cytoskeleton, G-and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA

Abbreviation

Beta-actin; ACTB

Synonyms

A26C1A; A26C1B; ACTB; ACTBB; Actin; Actin beta; Actin cytoplasmic 1; Actx; BRWS1; Beta cytoskeletal actin; Beta-actin; E430023M04Rik; MGC128179; N-terminally processed; PS1TP5 binding protein 1; PS1TP5BP1; b actin; cytoplasmic 1

UniProt

Q7ZVF9

Accession Number

Q7ZVF9

Expression System

E.coli

Tag

N-His

Sequence

Met1-Phe375

Applications

Immunogen

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 90% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

43.1 kDa

Observed Molecular Weight

42-48 kDa

Species

Danio rerio