Recombinant Rat FGF2 Protein (His Tag)

Specifications

Catalog number

PDER100150-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.

Abbreviation

FGF2

Synonyms

BFGF; Basic fibroblast growth factor; Basic fibroblast growth factor bFGF; FGF 2; FGF B; FGF-2; FGF2; FGF2 basic; FGFB; Fgf2; Fibroblast growth factor; Fibroblast growth factor 2; Fibroblast growth factor 2 (basic) ; HBGF 2; HBGF-2; HBGF2; HBGH 2; HBGH2; Heparin bindi; basic

UniProt

P13109

Accession Number

P13109-1

Expression System

E.coli

Tag

N-His

Sequence

Pro10-Ala144

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

15.2 kDa

Observed Molecular Weight

17.5 kDa

Species

Rat