Recombinant Rat PDGF-B Protein (His Tag)

Specifications

Catalog number

PDER100155-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 7, at sites where this gene and that for COL1A1 are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans resulting from unregulated expression of growth factor. Two alternatively spliced transcript variants encoding different isoforms have been identified for this gene.

Abbreviation

PDGF-B

Synonyms

Becaplermin; PDGF 2; PDGF B chain; PDGF subunit B; PDGF-2; PDGF2; PDGFB; Pdgfb; Platelet derived growth factor 2; Platelet-derived growth factor B chain; Platelet-derived growth factor beta polypeptide; Platelet-derived growth factor subunit B; Proto-oncogene c-Sis; S

UniProt

Q05028

Accession Number

Q05028-1

Expression System

E.coli

Tag

N-His

Sequence

Ser74-Thr182

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 85% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

12.2 kDa

Observed Molecular Weight

14.5 kDa

Species

Rat