Recombinant Rat C4a protein (GST tag)

Specifications

Catalog number

PDER100169-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Non-enzymatic component of C3 and C5 convertases and thus essential for the propagation of the classical complement pathway. Covalently binds to immunoglobulins and immune complexes and enhances the solubilization of immune aggregates and the clearance of IC through CR1 on erythrocytes.

Abbreviation

C4a

Synonyms

Complement C4

UniProt

P08649

Accession Number

P08649

Expression System

E.coli

Tag

N-GST

Sequence

Asn678-Arg753

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 85% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

34.6 kDa

Observed Molecular Weight

42 kDa

Species

Rat