Recombinant Rat VWF protein (His tag)

Specifications

Catalog number

PDER100170-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Von Willebrand Factor (VWF) is a multimeric glycoprotein involved in hemostasis in blood, binds receptors on the surface of platelets and in connective tissue, thereby mediating the adhesion of platelets to sites of vascular injury. From studies it appears that VWF protein uncoils under these circumstances, decelerating passing platelets. VWF protein is deficient or defective in von Willebrand disease (VWD) and is involved in a large number of other diseases, including thrombosis, thrombotic thrombocytopenic purpura, Stroke, Heyde's syndrome, possibly hemolytic-uremic syndrome and so on.

Abbreviation

VWF

Synonyms

F8VWF; VWD; VWF; Von Willebrand Factor; vWF

UniProt

Q62935

Accession Number

Q62935

Expression System

E.coli

Tag

N-His

Sequence

Lys 49-Asn 230

Field of Research

Cancer; Cardiovascular; Stem Cells

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

19.9 kDa

Observed Molecular Weight

25 kDa

Species

Rat