Recombinant Rat NT-proBNP protein (GST tag)

Specifications

Catalog number

PDER100173-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Cardiac hormone that plays a key role in mediating cardio-renal homeostasis. May also function as a paracrine antifibrotic factor in the heart. Acts by specifically binding and stimulating NPR1 to produce cGMP, which in turn activates effector proteins that drive various biological responses. Likely involved in regulating the extracellular fluid volume and maintaining the fluid-electrolyte balance through natriuresis, diuresis, kaluresis and chloruresis

Abbreviation

NT-proBNP

Synonyms

ANFB_RAT; Brain natriuretic factor prohormone; Gamma-brain natriuretic peptide; Iso-ANP; Natriuretic peptides B; Nppb; preproBNP; proBNP

UniProt

P13205

Accession Number

P13205

Expression System

E.coli

Tag

N-GST

Sequence

His27-Arg76

Field of Research

Neuroscience

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

30.4 kDa

Observed Molecular Weight

30 kDa

Species

Rat