Recombinant Rat MMP-12 protein (His tag)

Specifications

Catalog number

PDER100181-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Matrix metalloproteinase-12 (MMP12) is a secreted protein.It contains 4 hemopexin repeats and belongs to the peptidase M10A family. MMP12 may be involved in tissue injury and remodeling and have significant elastolytic activity. It can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3.

Abbreviation

MMP-12

Synonyms

Macrophage metalloelastase; MME; MMP-12; Matrix metalloproteinase-12; Mme; Mmel; Mmp12

UniProt

Q63341

Accession Number

Q63341

Expression System

E.coli

Tag

N-His

Sequence

Ala 286-Phe 463

Field of Research

Cancer; Cardiovascular; Signal Transduction

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

19.5 kDa

Observed Molecular Weight

24 kDa

Species

Rat