Recombinant Rat Occludin/OCLN protein (His tag)

Specifications

Catalog number

PDER100187-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Rat Occludin is a 523 amino acid (aa), predicted molecular weight 59 kDa integral membrane protein that localizes within tight junctions of epithelial and endothelial cells. May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. May be involved in the organization of actin in endothelial cells. Defects in OCLN are the cause of band-like calcification with simplified gyration and polymicrogyria (BLCPMG), also known as pseudo-TORCH syndrome. BLCPMG is a neurologic disorder with characteristic clinical and neuroradiologic features that mimic intrauterine TORCH infection in the absence of evidence of infection. Affected individuals have congenital microcephaly, intracranial calcifications, and severe developmental delay.

Abbreviation

Occludin; OCLN

Synonyms

OCLN_RAT; Occludin

UniProt

Q6P6T5

Accession Number

Q6P6T5

Expression System

E.coli

Tag

N-His

Sequence

Lys266-Thr522

Field of Research

Signal Transduction; Cell biology

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

28.2 kDa

Observed Molecular Weight

40 kDa

Species

Rat