Recombinant Rat Adrenomedullin/ADM protein (His tag)

Specifications

Catalog number

PDER100204-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Adrenomedullin (ADM, also AM) is a secreted, monomeric, 6 kDa member of the Adrenomedullin family of molecules. It is widely expressed, being found in smooth muscle cells, endothelium, adrenal medulla chromaffin cells, fibroblasts and keratinocytes. ADM has multiple functions, including vasodilation, the maintenance of vascular integrity, and the suppression of inflammatory mediator secretion. The ADM preproprecursor is 185 amino acids (aa) in length. It contains a 21 aa signal sequence, a processed 20 aa peptide termed PAMP (aa 22-41), an N-terminal propeptide (aa 45-91), the ADM precursor (amidation is required for maturation) (aa 94 143), and a C-terminal propeptide (aa 150-185) . The ADM precursor with a terminal Gly147 circulates naturally with bioactive, mature amidated ADM (aa 94 143) .

Abbreviation

Adrenomedullin; ADM

Synonyms

ADM precursor; Adrenomedullin; PAMP; Preproadrenomedullin; ProAM N terminal 20 peptide; ProAM N20

UniProt

P43145

Accession Number

P43145

Expression System

E.coli

Tag

N-His

Sequence

Thr 21-Leu 185

Field of Research

Signal Transduction; Cardiovascular; Neuroscience; Cancer; Metabolism

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

18.0 kDa

Observed Molecular Weight

25 kDa

Species

Rat