Recombinant Rat CD80/B7-1 protein (His tag)

Specifications

Catalog number

PDER100212-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

The B-lymphocyte activation antigen B7-1 (referred to as B7), also known as CD80, is a member of cell surface immunoglobulin superfamily and is expressed on the surface of antigen-presenting cells including activated B cells, macrophages and dendritic cells. As costimulatory ligands, B7-1 which exists predominantly as dimer and the related protein B7-2, interact with the costimulatory receptors CD28 and cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) expressed on T cells, and thus constitute one of the dominant pathways that regulate T cell activation and tolerance, cytokine production, and the generation of CTL. The B7/CD28/CTLA4 pathway has the ability to both positively and negatively regulate immune responses. CD80 is thus regarded as promising therapeutic targets for autoimmune diseases and various carcinomas.

Abbreviation

CD80; B7-1

Synonyms

Activation B7-1 antigen; B7; CD80; Cd28l; Ly-53; Ly53; MIC17; T-lymphocyte activation antigen CD80; TSA1

UniProt

G3V671

Accession Number

G3V671

Expression System

E.coli

Tag

N-His

Sequence

Gly 41-Ala 262

Field of Research

Immunology; Stem Cells

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

24.3 kDa

Observed Molecular Weight

32 kDa

Species

Rat