Recombinant Rat RB1 protein (His tag)

Specifications

Catalog number

PDER100220-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Retinoblastoma 1 protein (RB-1, also retinoblastoma-associated protein, pp110, and p105-Rb) is a 110 kDa tumor suppressor gene and member of the retinoblastoma protein family. Rat RB-1 is 920 amino acids in length. The protein contains a Pocket domain (aa 366-763), which is comprised of two other domains, domain A (aa 366-572) and domain B (aa 632-763), and a “spacer” (aa 573-631) . The Pocket domain binds to threonine-phosphorylated domain C (aa 763-920), which thereby prevents interaction with heterodimeric E2F/DP transcription factor complexes. RB-1 is expressed in the retina. The underphosphorylated, active form of RB-1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Defects in RB-1 lead to the childhood cancer retinoblastoma.

Abbreviation

RB1

Synonyms

OSRC; RB; RB1; osteosarcoma; p105-Rb; pRb; pp110; retinoblastoma 1; retinoblastoma suspectibility protein; retinoblastoma-associated protein

UniProt

P33568

Accession Number

P33568

Expression System

E.coli

Tag

N-His

Sequence

Arg 229-Met 450

Field of Research

Epigenetics and Nuclear Signaling; Developmental Biology

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

24.3 kDa

Observed Molecular Weight

32 kDa

Species

Rat