Recombinant Rat CD146/MCAM protein (His tag)

Specifications

Catalog number

PDER100227-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Melanomia Cell Adhesion Molecule (MCAM), also known as CD146 and MUC18, is a putative Ig-superfamily adhesion molecule that is expressed on endothelial cells and a variety of tumor cells. MCAM is associated with tumor progression and metastasis and may be involved in embryonic neural development. An alternately spliced isoform of rat MCAM has a 40 residue deletion in the cytoplasmic domain.

Abbreviation

CD146; MCAM

Synonyms

CD146; Cell surface glycoprotein MUC18; Gicerin; MUC18; Mcam; Melanoma cell adhesion molecule; Melanoma-associated antigen MUC18

UniProt

Q9EPF2

Accession Number

Q9EPF2

Expression System

E.coli

Tag

N-His

Sequence

His 151-Val 350

Field of Research

Cancer; Cardiovascular; Immunology; Signal Transduction; Stem Cells

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

21.9 kDa

Observed Molecular Weight

30 kDa

Species

Rat