Recombinant Vaccinia virus VACV (A27L) protein (His tag)

Specifications

Catalog number

PDEV100010-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Structural protein involved in the envelopment of mature virion (MV) to form the wrapped virion (WV) . The wrapping consists of the addition of Golgi membranes to the mature virion. Participates in mature virion (MV) movement within the infected cell. May play an indirect role in MV-cell fusion.

Abbreviation

VACV

Synonyms

A27L

UniProt

P11258

Accession Number

P11258

Expression System

E.coli

Tag

N-His

Sequence

Met 1-Glu 110

Field of Research

Microbiology

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

12.0 kDa

Observed Molecular Weight

14 kDa

Species

Vaccinia virus