Recombinant SARS-CoV-2 Nucleocapsid Protein (His Tag)

Specifications

Catalog number

PDEV100012-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. N protein packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.

Abbreviation

Nucleocapsid

Synonyms

2019-nCoV coronavirus NP Protein;2019-nCoV novel coronavirus Nucleoprotein Protein;2019-nCoV np Protein

UniProt

P0DTC9

Accession Number

P0DTC9

Expression System

E.coli

Tag

N-His

Sequence

Met1-Ala419

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

49.4 kDa

Observed Molecular Weight

50-60 kDa

Species

SARS-CoV-2