Recombinant Human B7-H3/CD276 Protein (His Tag)

Specifications

Catalog number

PDMH100358-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

CD276, also known as B7-H3, is a member of the B7 superfamily with signature IgV and IgG regions in extracellular domains. It is a type I transmembrane protein and shares 20–27% amino acid identity with other B7 family members. B7-H3 is involved in the activation of T lymphocytes, and regulates murine bone formation. It is also reported that B7-H3 may play an important role in muscle-immune interactions, providing further evidence of the active role of muscle cells in local immunoregulatory processes. B7-H3 is expressed on T-cells, natural killer cells, and antigen presenting cells, as well as some non-immune cells, such as osteoblasts, fibroblasts, fibroblast-like synoviocytes and epithelial cells. High expression of B7-H3 in tumor vasculature also correlates with poor survival in patients, suggesting that it may play a role in tumor cell migration.

Abbreviation

B7-H3; CD276

Synonyms

B7 homolog 3; B7-H3; B7H34Ig-B7-H3; CD276; CD276 antigen; CD276 molecule; Costimulatory molecule

UniProt

Q5ZPR3

Accession Number

Q5ZPR3

Expression System

HEK293 Cells

Tag

C-His

Sequence

Leu29-Thr461

Field of Research

Cancer; immunology; Stem cells

Endotoxin

< 1.0 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

47.3 kDa

Observed Molecular Weight

60-80 kDa

Species

Human