Recombinant Human SerpinE1/PAI-1 Protein (His Tag)

Specifications

Catalog number

PDMH100362-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Serpins are a group of proteins with similar structures that were first identified as a set of proteins able to inhibit proteases. They are the largest and most diverse family of serine protease inhibitors which are involved in a number of fundamental biological processes such as blood coagulation, complement activation, fibrinolysis, angiogenesis, inflammation and tumor suppression and are expressed in a cell-specific manner. Serpin E1 is a secreted protein which belongs to the Serpin family. Serpin E1 acts as 'bait' for tissue plasminogen activator, urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis. Defects in SERPINE1 are characterized by abnormal bleeding due to Serpin E1 defect in the plasma. High concentrations of Serpin E1 have been associated with thrombophilia which is an autosomal dominant disorder in which affected individuals are prone to develop serious spontaneous thrombosis.

Abbreviation

SerpinE1; PAI-1

Synonyms

Endothelial Plasminogen Activator Inhibitor; PAI; PAI-1; PAI1; PLANH1; Plasminogen Activator Inhibitor 1; SERPINE1; Serpin E1

UniProt

P05121

Accession Number

P05121

Expression System

HEK293 Cells

Tag

C-His

Sequence

Val24-Pro402

Field of Research

Cell biology; Cardiovascular; Cancer; metabolism

Endotoxin

< 1.0 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

43.8 kDa

Observed Molecular Weight

48 kDa

Species

Human