Recombinant Human LILRA5 Protein (His Tag)

Specifications

Catalog number

PDMH100368-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Leukocyte Immunoglobulin-like Receptor Subfamily A Member 5 (LILRA5) is a member of the leukocyte immunoglobulin-like receptors (LILR), comprise a family of activating and inhibitory type immunoreceptors. LILRA5 consists of a 227 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 10 aa cytoplasmic tail. The ECD contains two Ig-like domains and the transmembrane segment contains a positively charged aspartic acid residue which may mediate its association with the signaling molecule, FcR common gamma chain. LILRA5 is expressed by monocytes, macrophages, and neutrophils. Cross-linking of LILRA5 on monocytes induces the expression of pro-inflammatory cytokines (IL-1beta, IL-6, TNF-alpha) as well as the anti-inflammatory IL-10. It can be detected in tissues of the hematopoietic system, including bone marrow, spleen, lymph node and peripheral leukocytes. Crosslink of ILT-11 on the surface of monocytes has been shown to induce calcium flux and secretion of several proinflammatory cytokines, which suggests the roles of this protein in triggering innate immune responses.

Abbreviation

LILRA5

Synonyms

CD85 antigen-like family member F; CD85F; CD85f; ILT-11; ILT11; Immunoglobulin-like transcript 11; LILRA5; LILRB7; LIR-9; LIR9; Leukocyte immunoglobulin-like receptor 9; Leukocyte immunoglobulin-like receptor subfamily A member 5

UniProt

A6NI73

Accession Number

A6NI73

Expression System

HEK293 Cells

Tag

C-His

Sequence

Gly42-Arg268

Field of Research

Immunology

Endotoxin

< 1.0 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

26.1 kDa

Observed Molecular Weight

35-40 kDa

Species

Human