Recombinant Human SIGLEC2/CD22 Protein (His Tag)

Specifications

Catalog number

PDMH100369-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins, one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules.

Abbreviation

SIGLEC2; CD22

Synonyms

B-cell receptor CD22; B-lymphocyte cell adhesion molecule; BL-CAM; CD22; CD22 antigenMGC130020; CD22 molecule; SIGLEC-2; SIGLEC2FLJ22814; Siglec-2; T-cell surface antigen Leu-14; sialic acid binding Ig-like lectin 2

UniProt

P20273

Accession Number

P20273

Expression System

HEK293 Cells

Tag

C-His

Sequence

Asp20-Arg687

Field of Research

Cancer; immunology; Stem cells

Endotoxin

< 1.0 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

76.2 kDa

Observed Molecular Weight

100-140 kDa

Species

Human