Recombinant Human PL/PNLIP protein (His tag)

Specifications

Catalog number

PDMH100387-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

PNLIP is an enzyme which belongs to the lipase family. Secreted from the pancreas, PNLIP is the primary lipase that hydrolyzes dietary fat molecules in the human digestive system, converting triglyceride substrates found in ingested oils to monoglycerides and free fatty acids. Bile salts secreted from the liver and stored in gallbladder are released into the duodenum where they coat and emulsify large fat droplets into smaller droplets, thus increasing the overall surface area of the fat, which allows the lipase to break apart the fat more effectively. The resulting monomers (2 free fatty acids and one 2-monoacylglycerol) are then moved by way of peristalsis along the small intestine to be absorbed into the lymphatic system by a specialized vessel called a lacteal.

Abbreviation

PL; PNLIP

Synonyms

PL) (PTL) (Pancreatic lipase) (EC 3.1.1.3) ; Pancreatic triacylglycerol lipase

UniProt

P16233

Accession Number

P16233

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met 1-Cys465

Field of Research

Signal Transduction; Cardiovascular; Cancer; metabolism

Endotoxin

< 1.0 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

51.0 kDa

Observed Molecular Weight

50 kDa

Species

Human