Recombinant Human Calnexin/CANX protein (His tag)

Specifications

Catalog number

PDMH100389-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Calnexin/CANX is a single-pass type I membrane protein which belongs to the calreticulin family. It consists of a large N-terminal calcium-binding lumenal domain, a single transmembrane helix and a short (90 residues), acidic cytoplasmic tail. The function of calnexin is to retain unfolded or unassembled N-linked glycoproteins in the endoplasmic reticulum. Calnexin is a calcium-binding protein that interacts briefly with newly synthesized glycoproteins in the endoplasmic reticulum. Calnexin may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. Calnexin seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. Calnexin dwindles with aging and might contribute to a cytoprotection in an array of human age-related diseases.

Abbreviation

Calnexin; CANX

Synonyms

CANX; Calnexin; IP90; Major Histocompatibility Complex Class I Antigen-Binding Protein p88; p90

UniProt

P27824

Accession Number

P27824

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met1-Pro481

Field of Research

Tags & Cell Markers; Neuroscience

Endotoxin

< 1.0 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

52.8 kDa

Observed Molecular Weight

60 kDa

Species

Human