Recombinant Human Ephrin-A5/EFNA5 protein (His tag)

Specifications

Catalog number

PDMH100397-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Ephrin-A5 (EFNA5) belongs to the ephrin family, contains 1 ephrin RBD (ephrin receptor-binding) domain. Ephrin-A5 is a cell surface GPI-bound ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. It binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The interaction of EFNA5 with EPHA5 also mediates communication between pancreatic islet cells to regulate glucose-stimulated insulin secretion. Cognate/functional ligand for EPHA7, their interaction regulates brain development modulating cell-cell adhesion and repulsion.

Abbreviation

Ephrin-A5; EFNA5

Synonyms

AL-1; EFNA5; EPH-related receptor tyrosine kinase ligand 7; EPLG7; Ephrin-A5; LERK-7; LERK7

UniProt

P52803

Accession Number

P52803

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met1-Asn203

Field of Research

Signal Transduction; Neuroscience

Endotoxin

< 1.0 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

32.7 kDa

Observed Molecular Weight

32 kDa

Species

Human