Recombinant Human ILT4/LILRB2 protein (His tag)

Specifications

Catalog number

PDMH100418-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Members of the immunoglobulin-like transcript (ILT) family are activating and inhibitory immunoreceptors whose genes are located same locus that encodes killer cell Ig-like receptors (KIR) . Leukocyte Immunoglobulin-Like Receptor Subfamily B Member 2 (LIR-2) is a type I transmembrane protein. LIR-2 is expressed primarily on monocytes and dendritic cells (DC) . Human LIR-2 is produced as a 598 amino acino acid precursor including a 21 aa signal sequence, a 440 aa extracellular domain (ECD), a 21 aa transmenbrane segment, and a 116 aa cytoplasmic domain. LIR-2 binds to Classical MHCI proteins. Ligation of LIR-2 incluces Tyr phosphorylation within its cytoplasmic ITIMs, a requirement for association with SHP-1. LIR-2 mediates tolerogenic DC-induced CD4+ T cell energy in vitro and in vivo.

Abbreviation

ILT4; LILRB2

Synonyms

CD85 Antigen-Like Family Member D; CD85d; ILT-4; Immunoglobulin-Like Transcript 4; L; LIR-2; Leukocyte Immunoglobulin-Like Receptor 2; Leukocyte Immunoglobulin-Like Receptor Subfamily B Member 2; MIR-10; Monocyte/Macrophage Immunoglobulin-Like Receptor 10

UniProt

Q8N423

Accession Number

Q8N423

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met1-Val461

Field of Research

Immunology

Endotoxin

< 1.0 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

50.6 kDa

Observed Molecular Weight

75 kDa

Species

Human