Recombinant Human PDL1/CD274 protein (His tag)

Specifications

Catalog number

PDMH100425-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

CD274, also known as B7-H1 or programmed death ligand 1 (PD-L1), is a 40 kD type I transmembrane protein and a member of the B7 family within the immunoglobulin receptor superfamily. Programmed death-1 ligand-1 (PD-L1, CD274, B7-H1) has been identified as the ligand for the immunoinhibitory receptor programmed death-1 (PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance. By binding to PD1 on activated T-cells and B-cells, PD-L1 may inhibit ongoing T-cell responses by inducing apoptosis and arresting cell-cycle progression. Accordingly, it leads to growth of immunogenic tumor growth by increasing apoptosis of antigen specific T cells and may contribute to immune evasion by cancers. PD-L1 thus is regarded as promising therapeutic target for human autoimmune disease and malignant cancers.

Abbreviation

PDL1; CD274

Synonyms

B7 Homolog 1; B7-H1; B7H1; CD274; PD-L1; PDCD1 Ligand 1; PDCD1L1; PDCD1LG1; PDL1; Programmed Cell Death 1 Ligand 1; Programmed Death Ligand 1

UniProt

Q9NZQ7

Accession Number

Q9NZQ7

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met1-Thr239

Field of Research

Cancer; immunology

Endotoxin

< 1.0 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

26.2 kDa

Observed Molecular Weight

36 kDa

Species

Human