Recombinant Mouse Selenoprotein P/SEPP1 protein (His tag)

Specifications

Catalog number

PDMM100197-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Selenoprotein P (SeP) is an extracellular, monomeric glycoprotein containing up to 10 selenocysteine residues in the polypeptide chain. It is ubiquitously expressed in mammalian tissues, and in human plasma it accounts for at least 40% of the total selenium concentration. SeP binds to heparin and cell membranes, and is associated with endothelial cells. SeP in human plasma protects against peroxynitrite-mediated oxidation and reduces phospholipid hydroperoxide in vitro, in accordance with the presumption that it has a function as an extracellular oxidant defense. Immunochemical assays have demonstrated that its concentration in plasma varies much with selenium intake, but other factors also have an influence.

Abbreviation

Selenoprotein P; SEPP1

Synonyms

Plasma selenoprotein P; SeP; Selenop; Selenoprotein P

UniProt

P70274

Accession Number

P70274

Expression System

HEK293 Cells

Tag

C-His

Sequence

Glu20–Asn380

Endotoxin

< 1.0 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

39.6 kDa

Observed Molecular Weight

60 kDa

Species

Mouse