Recombinant Mouse B2M/Beta-2-microglobulin Protein (His Tag)

Specifications

Catalog number

PDMM100199-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

B2M, also known as β2-Microglobulin or CDABP0092, is a component of MHC class I molecules found expression in all nucleated cells (excludes red blood cells) . The major function of MHC class I moleculesis is to display fragments of proteins from within the cell to T-cells and cells containing foreign proteins will be attacked. B2M (β2-Microglobulin) is a low molecular weight protein. It was demonstrated that B2M (β2-Microglobulin) was localized in the membranes of nucleated cells and was found to be associated with HL-A antigens. B2M (β2-Microglobulin) is present in free form in various body fluids and as a subunit of histocompatibility antigens on cell surfaces lateral to the α3 chain. Unlike α3, β2 has no transmembrane region. Directly above β2 lies the α1 chain, which itself is lateral to the α2. In the absence of B2M (β2 microglobulin), very limited amounts of MHC class I (classical and non-classical) molecules can be detected on the surface. In the absence of MHC class I, CD8 T cells, a subset of T cells involved in the development of acquired immunity cannot develop. Low levels of B2M (β2 microglobulin) can indicate non-progression of HIV.

Abbreviation

B2M; Beta-2-microglobulin

Synonyms

B2MG; Beta 2 Microglobin; Beta 2 microglobulin Precursor; Beta Chain of MHC Class 1 Proteins; Beta Chain of MHC class I molecules; Beta-2-Microglobulin; CDABP0092; Hdcma22p; IMD43

UniProt

P01887

Accession Number

P01887

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met 1-Met 119

Field of Research

Cancer

Endotoxin

< 1.0 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

10.8 kDa

Observed Molecular Weight

12 kDa

Species

Mouse