Recombinant Mouse Endoglin/CD105 protein (His tag)

Specifications

Catalog number

PDMM100215-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Endoglin, also known as CD105, is a type I&nbsp, homodimeric transmembrane glycoprotein with a large, disulfide-linked, extracellular region and a short, constitutively phosphorylated cytoplasmic tail. Endoglin contains an RGD tripeptide which is a key recognition structure in cellular adhesion, , suggesting a critical role for endoglin in the binding of endothelial cells to integrins and/or other RGD receptors. Endoglin is highly expressed on vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta. It is also found on activated monocytes, mesenchymal stem cells and leukemic cells of lymphoid and myeloid lineages. As an accessory receptor for the TGF-β superfamily ligands, endoglin binds TGF-β1 and TGF-β3 with high affinity not by itself but by associating with TGF-β type I&nbsp, I receptor (TβRII) and activates the downstream signal pathways. In addition, in human umbilical vein endothelial cells, ALK-1 is also a receptor kinase for endoglin threonine phosphorylation, and mutations in either of the two genes result in the autosomal-dominant vascular dysplasia, hereditary hemorrhagic telangiectasia (HHT) . Endoglin has been regarded as a powerful biomarker of neovascularization, and is associated with several solid tumor types.

Abbreviation

Endoglin; CD105

Synonyms

AI528660; AI662476; CD105; Endo; S-endoglin

UniProt

Q63961

Accession Number

Q63961

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met1-Lys580

Field of Research

Immunology; Cancer; Cardiovascular; Stem Cells

Endotoxin

< 1.0 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

63.7 kDa

Observed Molecular Weight

75 kDa

Species

Mouse