Recombinant Rat Lp-PLA2/Pla2g7 protein (His tag)

Specifications

Catalog number

PDMR100087-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Secretory phopholipase A2 is an enzyme that hydrolyses the Sn-2 ester bond of phospholipids, generating free fatty acids and lysophospholipids. Most secretory PLA2s are stored in cytoplasmic granules and are released in the extracellular environment on appropriate cell activation. Thus, they are present at higher concentration in the plasma and biologic fluids of patients with systemic inflammatory, autoimmune, or allergic disease, such as acute pancreatitis, rheumatoid arthritis, bronchial asthma, and allergic rhinitis. Also known as Lp-PLA2, PLA2G-VII is a plasma enzyme bound to lipoproteins: 80% bound to LDL, 15%-20% to HDL, and the remainder to VLDL (4-6) . It is produced in major by mature macrophages and activated platelets. In contrast to other classical sPLA2s, PLA2G VII has poor specificity toward Sn-2 long chain fatty acids, unless heavily oxidized, and undergoes the catalysis of its substrates in the aqueous phase rather than at the interfacial surface of lipids. Thus, it has high specificity for water-soluble phospholipids in plasma including oxidatively modified phospholipids and platelet-activating factor (PAF) . Because of the latter activity, it is also known as PAF acetylhydrolase (PAF-AH) .

Abbreviation

Lp-PLA2; Pla2g7

Synonyms

EC:3.1.1.47; Pla2g7; Platelet-activating factor acetylhydrolase; rCG_43740

UniProt

Q5M7T7

Accession Number

Q5M7T7

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met1-Asn440

Field of Research

Immunology

Endotoxin

< 1.0 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

48.3 kDa

Observed Molecular Weight

60 kDa

Species

Rat