Recombinant Mouse Ngfb Protein (His Tag)

Specifications

Catalog number

PDEM100338-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

NGF is the first member discovered in the Neurotrophin family, which includes brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), and neurotrophin-4 (NT-4) . These proteins belong to the cysteine-knot family of growth factors that assume stable dimeric structures. Mouse beta-NGF is a homodimer of two 120 amino acid polypeptides. It shares approximately 90% homology at the amino acid level with human beta-NGF and 95.8% with rat beta-NGF. NGF signaling has been shown to play an important role in neuroprotection and repair. β-NGF acts as a growth and differentiation factor for B lymphocytes, and enhances B-cell survival. It is a potent neurotrophic factor that signals through its receptor β-NGFR, and plays a crucial role in the development and preservation of the sensory and sympathetic nervous systems.

Abbreviation

Ngfb

Synonyms

Beta-nerve growth factor; Beta-NGF; NGF

UniProt

P01139

Accession Number

P01139

Expression System

E.coli

Tag

N-His

Sequence

Ser122-Gly241

Field of Research

Neuroscience; Developmental Biology; Cell Biology

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

13.1 kDa

Observed Molecular Weight

13 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Species

Mouse