NELF-B Recombinant Rabbit Monoclonal Antibody [JE77-56]

Catalog number HA722320

Price for HA722320

Size / count 100 µL

About this product

In molecular biology, the NELF (negative elongation factor) is a four-subunit protein complex (NELF-A, NELF-B, NELF-C/NELF-D, and NELF-E) that negatively impacts transcription by RNA polymerase II (Pol II) by pausing about 20-60 nucleotides downstream from the transcription start site (TSS). NELF is located in the nucleus. NELF binds in a stable complex with DSIF (5,6-dichloro-1-β-d-ribofuranosylbenzimidazole (DRB)-sensitivity inducing factor) and RNA polymerase II together, but not with either alone. Due to its role in transcription, NELF is also a key player in the negative function of DSIF. NELF also works with DSIF to inhibit the speed of Pol II during the elongation phase in transcription. In D. melanogaster, the HSP70 gene is affected by NELF and DSIF through the induction of promoter proximal pausing. It is thought that NELF arose to assist DSIF by amplifying its negative effects in order to increase gene expression control. P-TEFb (positive transcription elongation factor b) inhibits the effect of NELF and DSIF on Pol II elongation, via its phosphorylation of serine-2 of the C-terminal domain of Pol II, and the SPT5 subunit of DSIF, causing dissociation of NELF.

Specifications

Catalog number: HA722320
Size / count: 100 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9000-83-3
Product Name Alternative: A730008L03Rik antibody AB041607 antibody AI663983 antibody Cobra1 antibody Cofactor of BRCA1 antibody DKFZp586B0519 antibody KIAA1182 antibody Negative elongation factor B antibody Negative elongation factor protein B antibody Nelf b antibody NELF-B antibody NELFB antibody RGD1307832 antibody RP13-122B23.3 antibody RP23-132N23.4 antibody
Abbreviation: NELFB, COBRA1, KIAA1182
Swiss Prot: Q8WX92 Human, Q8C4Y3 Mouse,
Cellular Locus: Nucleus.
Host: Rabbit
Species Reactivity: Human, Mouse, Rat, Monkey
Immunogen: Recombinant protein within Human NELF-B aa 1-250 / 580.
Isotype: IgG
Clone: JE77-56
Conjugation: Non-conjugated
Type: Recombinant Rabbit monoclonal Antibody
Applications: WB
Positive Control: T-47D cell lysate, HeLa cell lysate, BxPC-3 cell lysate, HepG2 cell lysate, MCF7 cell lysate, COS-1 cell lysate, NIH/3T3 cell lysate, Neuro-2a cell lysate, C2C12 cell lysate, C6 cell lysate, L6 cell lysate, PC-12 cell lysate.
Concentration: 1 mg/mL
Dilution: WB: 1:1,000
Purity: Protein A affinity purified.
Form: Liquid
Buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight: Predicted band size: 66 kDa
Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
Recombinant Antibody: Yes

Frequently Asked Questions

What is the catalog number for NELF-B Recombinant Rabbit Monoclonal Antibody [JE77-56]?

The catalog number for NELF-B Recombinant Rabbit Monoclonal Antibody [JE77-56] is HA722320.

What sizes are available for NELF-B Recombinant Rabbit Monoclonal Antibody [JE77-56]?

NELF-B Recombinant Rabbit Monoclonal Antibody [JE77-56] is available in the following size(s): 100 µL.

Is NELF-B Recombinant Rabbit Monoclonal Antibody [JE77-56] in stock?

NELF-B Recombinant Rabbit Monoclonal Antibody [JE77-56] is currently made to order / not held in immediate stock. Contact us for lead time.

What is NELF-B Recombinant Rabbit Monoclonal Antibody [JE77-56] used for?

What expression system was used for this recombinant protein?

The expression system, such as E. coli, HEK293, or insect cells, is listed on the datasheet under production details, which can affect post-translational modifications relevant to your application.

Can I return this product if I ordered the wrong one?

As a consumer, you have a 14-day right of withdrawal from receipt under EU consumer protection law, submitted via our withdrawal/cancellation request form. Note this applies to unused, undamaged products, and doesn’t override the expiry/storage conditions on performance-based claims.

Is this product tested for endotoxin levels?

Endotoxin testing status is noted on the datasheet where applicable, particularly for recombinant proteins intended for cell-based or functional assays. If this isn’t listed and you need confirmation, contact support with the catalogue number.