Mini Sample Mouse CD23 (Receptor II for the Fc Region of Immunoglobulin E) ELISA Kit

Specifications

Catalog number

E-MSEL-M0121-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

CD23

UniProt

P20693

Cross Reactivity

This kit recognizes CD23 in samples.No significant cross-reactivity or interference between CD23 and analogues was observed.

Target

FCER2; BLAST-2; CD23A; CLEC4J; FCE2; IGEBF; Fc epsilon RII Recommended name:Low affinity immunoglobulin epsilon Fc receptor Alternative names:Fc-epsilon-RII Lymphocyte IgE receptor

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cell Biology

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to CD23 . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for CD23 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain CD23 , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 46 nm. The OD value is proportional to the concentration of CD23 . You can calculate the concentration of CD23 in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

78.13-5000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

46.88 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Detection Instrument

Microplate reader

Species

Mouse

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Mouse CD23 concentrations in serum, plasma and other biological fluids.