QuicKey Pro Rat PCSK9 (Proprotein convertase subtilisin/kexin type 9) ELISA Kit

Specifications

Catalog number

E-OSEL-R0016-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

PCSK9

UniProt

P59996

Cross Reactivity

This kit recognizes PCSK9 in samples.No significant cross-reactivity or interference between PCSK9 and analogues was observed.

Target

FH3; HCHOLA3; LDLCQ1; NARC-1; NARC1; PC9

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Signal Transduction; Metabolism; Neuroscience

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to PCSK9 . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for PCSK9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PCSK9 , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 24 nm. The OD value is proportional to the concentration of PCSK9 . You can calculate the concentration of PCSK9 in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

1 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.78-50 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.47 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,6 months

Detection Instrument

Microplate reader

Species

Rat

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Rat PCSK9 concentrations in serum, plasma and other biological fluids.