Uncoated Mouse TNFSF8 (Tumor necrosis factor ligand superfamily member 8) ELISA Kit

Specifications

Catalog number

E-UNEL-M0190-01

Size / count

5x 96 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

TNFSF8

UniProt

P32972

Cross Reactivity

This kit recognizes Mouse TNFSF8 in samples.No significant cross-reactivity or interference between Mouse TNFSF8 and analogues was observed

Target

TNFSF; Cd30l; Cd30lg; Tnfsf8; TNFSF8; CD153; CD30 ligand; Tumor necrosis factor ligand superfamily member 8

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Immunology

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TNFSF8. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TNFSF8 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TNFSF8, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human TNFSF8. You can calculate the concentration of Human TNFSF8 in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

24 h

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.31-20 ng/mL

Precision

Both intra-CV and inter-CV are < 10%

Recovery

80%-120%

Shipping Conditions

Ice bag

Storage Conditions

-20°C,12 months

Species

Mouse

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Mouse TNFSF8 concentrations in serum, plasma and other biological fluids.