Uncoated cAMP (Cyclic adenosine monophosphate) ELISA Kit

Specifications

Catalog number

E-UNEL-0001-01

Size / count

5x 96 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

CAMP

Cross Reactivity

This kit recognizes cAMP in samples.No significant cross-reactivity or interference between cAMP and analogues was observed

Target

3'-5'-cyclic adenosine monophosphate; CAMP; Cyclic AMP; Cyclic adenosine monophosphate

Type

Competitive-ELISA

Applications

ELISA

Field of Research

Signal Transduction

Assay Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with cAMP. During the reaction, cAMP in the sample or standard competes with a fixed amount of cAMP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to cAMP. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of cAMP in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Assay Performance Time

24 h

Sample Type

Serum, plasma and other biological fluids

Detection Range

1.56-100 ng/mL

Precision

Both intra-CV and inter-CV are < 10%

Recovery

80%-120%

Shipping Conditions

Ice bag

Storage Conditions

-20°C,12 months

Species

Universal

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of cAMP concentrations in serum, plasma and other biological fluids.