Purified Anti-Mouse IL-10 Antibody[JES5-16E3]

Specifications

Catalog number

E-AB-F1197A-01

Size / count

25 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha. Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation.

Abbreviation

IL-10

UniProt

P18893

Host

Rat

Reactivity

Mouse

Immunogen

Recombinant Mouse IL-10 protein

Target

Interleukin; IL-; CSIF; If2a; Il-10; IL10A; TGIF; Interleukin-10; IL10; Cytokine synthesis inhibitory factor; N/A; CSIF; If2a; Il-10

Clonality

Monoclonal

Clone

JES5-16E3

Conjugation

Unconjugated

Applications

FCM

Purification

>98%, Protein A/G purified

Concentration

≥ 1 mg/mL

Dilution

66

Buffer

PBS, pH 7.2. Contains 0.05% proclin 300.

Shipping Conditions

Ice bag

Storage Conditions

Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles.

Isotype

Rat IgG2b, κ