CCNE1/Cyclin-E1 Monoclonal Antibody

Specifications

Catalog number

AN200193P

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

Background

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB.

Abbreviation

CCNE1

UniProt

P24864

Host

Mouse

Reactivity

Human

Immunogen

Recombinant Human CCNE1/Cyclin-E1 protein

Target

G1/S-specific cyclin-E; pCCNE; cyclin E; CCNE1; CCNE; pCCNE1; cyclin E1; cyclin E variant ex5del; cyclin E variant ex7del; Cyclin Es; Cyclin Et; CyclinE; G1/S specific cyclin E; G1/S-specific cyclin-E1

Clonality

Monoclonal

Clone

11D10

Conjugation

Unconjugated

Applications

IHC-P

Field of Research

Cell Biology; Epigenetics and Nuclear Signaling; Cancer

Purification

Protein A

Concentration

1 mg/mL

Dilution

IHC-P 1:50-1:200

Buffer

0.2 μm filtered solution in PBS

Shipping Conditions

Ice bag

Storage Conditions

This antibody can be stored at 2°C-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Isotype

IgG1