Recombinant Osteopontin/SPP1/ETA-1 Monoclonal Antibody

Specifications

Catalog number

AN300326P-01

Size / count

20 µL

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Osteopontin (OPN, previously also referred to as transformation-associated secreted phosphoprotein, bone sialoprotein I, 2ar, 2B7, early T lymphocyte activation 1 protein, minopotin, calcium oxalate crystal growth inhibitor protein), is a secreted, highly acidic, calcium-binding, RGD-containing, phosphorylated glycoprotein originally isolated from bone matrix. Subsequently, OPN has been found in kidney, placenta, blood vessels and various tumor tissues. Many cell types (including macrophages, osteoclasts, activated T-cells, fibroblasts, epithelial cells, vascular smooth muscle cells, and natural killer cells) can express OPN in response to activation by cytokines, growth factors or inflammatory mediators. Elevated expression of OPN has also been associated with numerous pathobiological conditions such as atherosclerotic plaques, renal tubulointerstitial fibrosis, granuloma formations in tuberculosis and silicosis, neointimal formation associated with balloon catheterization, metastasizing tumors, and cerebral ischemia. Human OPN cDNA encodes a 314 amino acid (aa) residue precursor protein with a 16 aa residue predicted signal peptide that is cleaved to yield a 298 aa residue mature protein with an integrin binding sequence (RGD), and N- and O-glycosylation sites. By alternative splicing, at least three human OPN isoforms exist. OPN has been shown to bind to different cell types through RGD-mediated interaction with the integrins alpha v beta 1, alpha v beta 3, alpha v beta 5, and non-RGD-mediated interaction with CD44 and the integrins alpha 8 beta 1 or alpha 9 beta 1. OPN exists both as a component of extracellular matrix and as a soluble molecule. Functionally, OPN is chemotactic for macrophages, smooth muscle cells, endothelial cells and glial cells. OPN has also been shown to inhibit nitric oxide production and cytotoxicity by activated macrophages. Human, mouse, rat, pig and bovine OPN share from approximately 40% - 80% amino acid sequence identity. Osteopontin is a substrate for proteolytic cleavage by thrombin, enterokinase, MMP-3 and MMP-7. The functions of OPN in a variety of cell types were shown to be modified as a result of proteolytic cleavage.

Abbreviation

Spp1

UniProt

P10451

Host

Rabbit

Reactivity

Human

Immunogen

Recombinant Human Osteopontin/SPP1/ETA-1 protein

Target

BSP; Bone sialoprotein; secreted phosphoprotein; Early T-Lymphocyte Activation; PSEC; SPP; ETA; BNSP; BSPI; ETA-1; OPN; Osteopontin; SPP1; Bone Sialoprotein I; BSP1; Early T-Lymphocyte Activation 1; SPP-1; Nephropontin; Uropontin; Bone sialoprotein 1; ETA 1; secreted phosphoprotein 1; SPP 1; Urinary stone protein; PSEC0156

Clonality

Monoclonal

Clone

9F5

Conjugation

Unconjugated

Applications

IHC-P; FCM

Field of Research

Cardiovascular; Signal Transduction; Stem Cells; Cancer

Purification

Protein A

Concentration

1 mg/mL

Dilution

IHC-P 1:1000-1:4000; FCM 1:25-1:100

Buffer

0.2 μm filtered solution in PBS

Shipping Conditions

Ice bag

Storage Conditions

This antibody can be stored at 2°C-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Isotype

IgG