Recombinant Phospho-Histone H2A.X (Ser139) Monoclonal Antibody

Specifications

Catalog number

AN300358L-01

Size / count

20 µL

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.

Abbreviation

H2AX

UniProt

P16104

Host

Rabbit

Reactivity

Human

Immunogen

A synthetic peptide corresponding to residues around Ser139 of Human Phospho-Histone H2A.X

Target

H2A.X; H2A/X; H2AX; Histone H2AX; H2AFX; gamma H2A.X; γH2AX

Clonality

Monoclonal

Clone

12G4

Conjugation

Unconjugated

Applications

WB; IHC-P

Field of Research

Epigenetics and Nuclear Signaling

Purification

Protein A

Concentration

1 mg/mL

Dilution

WB 1:2000-1:20000; IHC-P 1:1000-1:5000

Buffer

10 mM sodium HEPES, 150 mM NaCl, 100 μg/mL protein protectant, 50% glycerol, pH 7.5

Shipping Conditions

Ice bag

Storage Conditions

This antibody can be stored at 2°C-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

15 kDa

Observed Molecular Weight

15 kDa

Isotype

IgG