Recombinant Transferrin Receptor/TFRC/CD71 Monoclonal Antibody

Specifications

Catalog number

AN300360P-01

Size / count

20 µL

Dry ice

No

Hazardous

No

Discontinued

No

Background

The Transferrin Receptor (TfR or TfR-1, designated CD71) is a type 2 transmembrane glycoprotein expressed on erythroid progenitors, muscle cells and proliferating cells as a 188 kDa disulfide-linked homodimer of 95 kDa monomers (1‑4) . As the major mediator of cellular iron uptake, it binds and internalizes diferric transferrin, allowing iron release at the low pH of the endosome. The human TfR cDNA encodes 760 amino acids (aa) including a 67 aa N-terminal intracellular domain, a 21 aa transmembrane domain, and a 672 aa extracellular domain (ECD) with helical, peptidase (nonfunctional), and ligand binding domains, including an RGD potential integrin binding site. Human TfR ECD shares 75‑80% aa identity with mouse, rat, feline, canine, equine, porcine and bovine TfR. A 679 aa alternately spliced form begins at aa 82 and is presumably secreted, while in an 804 aa form, 44 aa are inserted at aa 518 within the peptidase region. Most soluble TfR (sTfR) arises from trypsin proteolysis at aa 100, producing the circulating form of TfR. sTfR concentration in plasma or serum is proportional to total TfR and can be increased by iron deficiency. Erythroid progenitors, which use iron for hemoglobin synthesis, normally account for the bulk of total body TfR production. Since rapidly growing cells require iron to replicate DNA, cancer cells can express up to 5-fold more TfR than quiescent cells in the surrounding tissue. Antibody targeting of TfR can inhibit tumor cell proliferation and induce apoptosis. The hereditary hemochromatosis protein HFE competes with diferric transferrin for binding to TfR, and targets TfR for degradation rather than recycling. TfR has been reported to have ferritin-independent functions in T cell development, immunological synapse formation and galectin-3-mediated cell death, and to be a cell entry receptor for New World hemorrhagic fever arenaviruses.

Abbreviation

TFRC

UniProt

P02786

Host

Rabbit

Reactivity

Human

Immunogen

Recombinant Human Transferrin Receptor/TFRC/CD71 protein

Target

IMD; sTfR; transferrin receptor protein; TFRC; CD71; IMD46; T9; TFR; TFR1; TR; TRFR; p90; transferrin receptor; sTfR1; transferrin receptor protein 1; T9

Clonality

Monoclonal

Clone

12G8

Conjugation

Unconjugated

Applications

WB; IP

Field of Research

Cardiovascular; Immunology; Stem Cells; Cancer; Metabolism

Purification

Protein A

Concentration

1 mg/mL

Dilution

WB 1:500-1:2000; IP 0.5-2 μL/mg of lysate

Buffer

0.2 μm filtered solution in PBS

Shipping Conditions

Ice bag

Storage Conditions

This antibody can be stored at 2°C-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

84 kDa

Observed Molecular Weight

95 kDa

Isotype

IgG