Recombinant Phospho-p38 MAPK (Thr180, Tyr182) Monoclonal Antibody

Specifications

Catalog number

AN300364L-01

Size / count

20 µL

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.

Abbreviation

MAPK14

UniProt

Q16539

Host

Rabbit

Reactivity

Human

Immunogen

A synthetic phosphopeptide corresponding to residues around Thr180, Tyr182 of human Phospho-p38 MAPK.

Target

Mxi; MAPK; CSPB; MAP kinase MXI; MAX-interacting protein; Mitogen-activated protein kinase; MAPK14; CSBP; CSBP1; CSBP2; CSPB1; EXIP; Mxi2; PRKM14; PRKM15; RK; SAPK2A; p38; p38ALPHA; p38 MAPK; MAP kinase MXI2; MAX-interacting protein 2; Mitogen-activated protein kinase 14; CSAID binding protein; CSAID Binding Protein 1; CSAID-binding protein; Csaids binding protein; CSBP 1; CSBP 2; Cytokine suppressive anti-inflammatory drug-binding protein; MAP kinase 14; MAP kinase p38 alpha; MAPK 14; MAX interacting protein 2; Mitogen Activated Protein Kinase 14; Mitogen activated protein kinase p38 alpha; Mitogen-activated protein kinase p38 alpha; MK14; Mxi 2; p38 ALPHA; p38 MAP kinase; p38 mitogen activated protein kinase; p38alpha Exip; p38-α

Clonality

Monoclonal

Clone

12G13

Conjugation

Unconjugated

Applications

WB; IHC-P; ICC/IF

Field of Research

Signal Transduction; Immunology

Purification

Protein A

Concentration

1 mg/mL

Dilution

WB 1:1000-1:10000; IHC-P 1:200-1:1000; ICC/IF 1:20-1:100

Buffer

10 mM sodium HEPES, 150 mM NaCl, 100 μg/mL protein protectant, 50% glycerol, pH 7.5

Shipping Conditions

Ice bag

Storage Conditions

This antibody can be stored at 2°C-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

41 kDa

Observed Molecular Weight

40 kDa

Isotype

IgG