Recombinant Phospho-S6 Ribosomal Protein (Ser240, 244) Monoclonal Antibody

Specifications

Catalog number

AN300371L-01

Size / count

20 µL

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit. The protein belongs to the S6E family of ribosomal proteins. It is the major substrate of protein kinases in the ribosome, with subsets of five C-terminal serine residues phosphorylated by different protein kinases. Phosphorylation is induced by a wide range of stimuli, including growth factors, tumor-promoting agents, and mitogens. Dephosphorylation occurs at growth arrest. The protein may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

Abbreviation

RPS6

UniProt

P62753

Host

Rabbit

Reactivity

Human

Immunogen

A synthetic peptide corresponding to residues around

Target

RPS; OK/SW-cl; RPS6; S6; OK/SW-cl.2

Clonality

Monoclonal

Clone

11A9

Conjugation

Unconjugated

Applications

WB; IHC-P

Field of Research

Epigenetics and Nuclear Signaling

Purification

Protein A

Concentration

1 mg/mL

Dilution

WB 1:1000-1:5000; IHC-P 1:1000-1:5000

Buffer

10 mM sodium HEPES, 150 mM NaCl, 100 μg/mL protein protectant, 50% glycerol, pH 7.5

Shipping Conditions

Ice bag

Storage Conditions

This antibody can be stored at 2°C-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

28 kDa

Observed Molecular Weight

35 kDa

Isotype

IgG