Recombinant Phospho-Chk1 (Ser317) Monoclonal Antibody

Specifications

Catalog number

AN300380L-01

Size / count

20 µL

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene.

Abbreviation

CHEK1

UniProt

O14757

Host

Rabbit

Reactivity

Human

Immunogen

A synthetic phosphopeptide corresponding to residues around Ser317 of human Chk1.

Target

CHK; CHEK; CHEK1; CHK1

Clonality

Monoclonal

Clone

11G3

Conjugation

Unconjugated

Applications

WB; IHC-P

Field of Research

Epigenetics and Nuclear Signaling; Cancer

Purification

Protein A

Concentration

1 mg/mL

Dilution

WB 1:1000-1:2000; IHC-P 1:500-1:2000

Buffer

10 mM sodium HEPES, 150 mM NaCl, 100 μg/mL protein protectant, 50% glycerol, pH 7.5

Shipping Conditions

Ice bag

Storage Conditions

This antibody can be stored at 2°C-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

54 kDa

Observed Molecular Weight

58 kDa

Isotype

IgG