Recombinant ORP150/ORP150/HYOU1/HSP12A Monoclonal Antibody

Specifications

Catalog number

AN300399P

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

Background

The protein encoded by this gene belongs to the heat shock protein 70 family. This gene uses alternative transcription start sites. A cis-acting segment found in the 5' UTR is involved in stress-dependent induction, resulting in the accumulation of this protein in the endoplasmic reticulum (ER) under hypoxic conditions. The protein encoded by this gene is thought to play an important role in protein folding and secretion in the ER. Since suppression of the protein is associated with accelerated apoptosis, it is also suggested to have an important cytoprotective role in hypoxia-induced cellular perturbation. This protein has been shown to be up-regulated in tumors, especially in breast tumors, and thus it is associated with tumor invasiveness. This gene also has an alternative translation initiation site, resulting in a protein that lacks the N-terminal signal peptide. This signal peptide-lacking protein, which is only 3 amino acids shorter than the mature protein in the ER, is thought to have a housekeeping function in the cytosol. In rat, this protein localizes to both the ER by a carboxy-terminal peptide sequence and to mitochondria by an amino-terminal targeting signal. Alternative splicing results in multiple transcript variants.

Abbreviation

HYOU1

UniProt

Q9Y4L1

Host

Rabbit

Reactivity

Human

Immunogen

Recombinant Human ORP150/HYOU1/HSP12A protein

Target

HYOU; Hypoxia up-regulated protein; Grp; ORP; HYOU1; GRP-170; Grp170; HSP12A; ORP-150; ORP150;150 kDa oxygen-regulated protein;170 kDa glucose-regulated protein; Hypoxia up-regulated protein 1

Clonality

Monoclonal

Clone

9D5

Conjugation

Unconjugated

Applications

WB; IHC-P; ICC/IF; IP

Field of Research

Cardiovascular; Signal Transduction; Neuroscience; Epigenetics and Nuclear Signaling; Cancer; Metabolism

Purification

Protein A

Concentration

1 mg/mL

Dilution

WB 1:500-1:2000; IHC-P 1:100-1:500; ICC/IF 1:20-1:100; IP 1-4 μL/mg of lysate

Buffer

0.2 μm filtered solution in PBS

Shipping Conditions

Ice bag

Storage Conditions

This antibody can be stored at 2°C-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

111 kDa

Observed Molecular Weight

170 kDa

Isotype

IgG