Recombinant AK4/Adenylate Kinase 4/AK3L1 Monoclonal Antibody

Specifications

Catalog number

AN300420P

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Adenylate kinase isoenzyme 4, mitochondrial, also known as ATP-AMP transphosphorylase, Adenylate kinase 3-like, AK4 and AK3L1, is a member theadenylate kinase family. AK4/AK3L1 is localized to the mitochondrial matrix. Adenylate kinases regulate the adenine and guanine nucleotide compositions within a cell by catalyzing the reversible transfer of phosphate group among these nucleotides. Five isozymes of adenylate kinase have been identified in vertebrates. Expression of these isozymes is tissue-specific and developmentally regulated. AK4/AK3L1 catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. It may also be active with GTP. Adenylate kinase 4 (AK4/AK3L1) is a unique member with no enzymatic activity in the adenylate kinase (AK) family although it shares high sequence homology with other AKs. It remains unclear what physiological function AK4 might play or why it is enzymatically inactive. AK4/AK3L1 retains the capability of binding nucleotides. It has a glutamine residue instead of a key arginine residue in the active site well conserved in other AKs. The enzymatically inactive AK4 is a stress responsive protein critical to cell survival and proliferation. AK4/AK3L1 is likely that the interaction with the mitochondrial inner membrane protein ANT is important for AK4 to exert the protective benefits to cells under stress. AK4/AK3L1 also acts on the specific mechanism of energy metabolism rather than control of the homeostasis of the ADP pool ubiquitously.

Abbreviation

AK4

UniProt

P27144

Host

Rabbit

Reactivity

Human

Immunogen

Recombinant Human AK4/AK3L1 protein

Target

AK3L2; AK3; AK3L1; AK4

Clonality

Monoclonal

Clone

3G5

Conjugation

Unconjugated

Applications

WB; ICC/IF; IP

Field of Research

Signal Transduction; Metabolism

Purification

Protein A

Concentration

1 mg/mL

Dilution

WB 1:500-1:2000; ICC/IF 1:20-1:100; IP 1-4 μL/mg of lysate

Buffer

0.2 μm filtered solution in PBS

Shipping Conditions

Ice bag

Storage Conditions

This antibody can be stored at 2°C-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

26 kDa

Observed Molecular Weight

26 kDa

Isotype

IgG