Recombinant PSGL-1/CD162/SELPLG Monoclonal Antibody

Specifications

Catalog number

AN300442P

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

Background

P-selectin glycoprotein ligand-1 (PSGL-1), also known as SELPLG or CD162, is the high affinity ounter-receptor for P-selectin on expressed on activated endothelial cells and platelets. PSGL-1 is a mucin-type glycoprotein, expressed on leukocytes and platelets as a homodimer of two disulfide-linked subunits of ~12 kD. As cell adhesion molecules, multiple studies have shown that PSGL-1/ P-selectin interaction is required for the normal recruitment of leukocytes during inflammatory reactions, and also participates in hemostatic responses. PSGL-1 protein requires two distinct posttranslational modifications for the Ca2+-dependent recognition by the lectin domain of P-selectin, that is tyrosine sulfation and specific O-linked glycosylation (sialic acid and fucose) . PSGL-1 can also bind to other two members of the selectin family, E-selectin (endothelial) and L-selectin (leukocyte), but binds best to P-selectin.

Abbreviation

SELPLG

UniProt

Q14242

Host

Rabbit

Reactivity

Human

Immunogen

Recombinant Human PSGL-1/CD162/SELPLG Protein

Target

P selectin glycoprotein ligand; P-selectin glycoprotein ligand; CD 162; CD162; CD162 antigen; CLA; Cutaneous lymphocyte associated associated antigen; P selectin glycoprotein ligand 1; P selectin glycoprotein ligand 1 precursor; P-selectin glycoprotein ligand 1; PSGL 1; PSGL1; PSGL-1; Selectin P ligand; SELPL; SELPLG; PEN-5

Clonality

Monoclonal

Clone

2D3

Conjugation

Unconjugated

Applications

IHC-P; FCM

Field of Research

Immunology; Cardiovascular

Purification

Protein A

Concentration

1 mg/mL

Dilution

IHC-P 1:100-1:500; FCM 1:25-1:100

Buffer

0.2 μm filtered solution in PBS

Shipping Conditions

Ice bag

Storage Conditions

This antibody can be stored at 2°C-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Isotype

IgG