Recombinant P4HB Monoclonal Antibody

Specifications

Catalog number

AN300541P

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

Background

Protein disulfide-isomerase, also known as Cellular thyroid hormone-binding protein, Prolyl 4-hydroxylase subunit beta, p55 and P4HB, is a peripheral membrane protein that belongs to the protein disulfide isomerase family. P4HB is highly abundant. In some cell types, it seems to be also secreted or associated with the plasma membrane, where it undergoes constant shedding and replacement from intracellular sources. P4HB localizes near CD4-enriched regions on lymphoid cell surfaces. It is identified by mass spectrometry in melanosome fractions from stage I to stage IV. P4HB reduces and may activate fusogenic properties of HIV-1 gp12 surface protein, thereby enabling HIV-1 entry into the cell. P4HB catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, it seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. P4HB may therefore cause structural modifications of exofacial proteins. Inside the cell, it seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, P4HB functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, it facilitates aggregation (anti-chaperone activity) . P4HB may be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. It also acts as a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP.

Abbreviation

P4HB

UniProt

P09103

Host

Rabbit

Reactivity

Mouse

Immunogen

Recombinant Mouse P4HB protein

Target

PDIA; Pdia1; P4hb; Cellular thyroid hormone-binding protein; ERBA2L; ERp59; p55; PDI; PO4DB; Prolyl 4-hydroxylase subunit beta; Thbp

Clonality

Monoclonal

Clone

3F9

Conjugation

Unconjugated

Applications

IHC-P; ICC/IF

Purification

Protein A

Concentration

1 mg/mL

Dilution

IHC-P 1:100-1:500; ICC/IF 1:20-1:100

Buffer

0.2 μm filtered solution in PBS

Shipping Conditions

Ice bag

Storage Conditions

This antibody can be stored at 2°C-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Isotype

IgG