Recombinant CD3D & CD3E Monoclonal Antibody

Specifications

Catalog number

AN300594P-01

Size / count

20 µL

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

The CD3 complex, composed of gamma, delta, epsilon, and zeta subunits, is essential for the assembly, trafficking, and surface expression of the T cell receptor (TCR) complex. These subunits are structurally related members of the immunoglobulin superfamily and are encoded by closely linked genes on human chromosome 11. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells, but not on B or NK cells. The CD3 subunits play a crucial role in transducing antigen-recognition signals into the cytoplasm of T cells. The cytoplasmic tails of CD3 subunits contain a double tyrosine-based motif that associates with cytoplasmic signal transduction molecules, mediating T cell activation through the TCR. Crosslinking of the TCR initiates intracellular biochemical pathways that result in cellular activation, proliferation, and potentially growth arrest and cell survival. CD3 is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes, and Purkinje cells in the cerebellum. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. Defects in the CD3 gene are associated with CD3 immunodeficiency, highlighting its importance in immune function and regulation.

Abbreviation

CD3D & CD3E

UniProt

P07766&P04234

Host

Rabbit

Reactivity

Human

Immunogen

Recombinant Human CD3D & CD3E Heterodimer protein

Target

IMD; CD3E; IMD18; T3E; TCRE; CD3e molecule; CD3 epsilon; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface antigen T3/Leu-4 epsilon chain; T3; CD_antigen: CD3e; epsilon

Clonality

Monoclonal

Clone

12B12

Conjugation

Unconjugated

Applications

IHC-P; FCM

Field of Research

Immunology; Stem Cells

Purification

Protein A

Concentration

1 mg/mL

Dilution

IHC-P 1:100-1:500; FCM 1:25-1:100

Buffer

0.2 μm filtered solution in PBS

Shipping Conditions

Ice bag

Storage Conditions

This antibody can be stored at 2°C-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Isotype

IgG