Recombinant Histone H2B (Acetyl Lys46) Monoclonal Antibody

Specifications

Catalog number

AN301406L-01

Size / count

50 µL

Dry ice

No

Hazardous

No

Discontinued

No

Background

Histone post-translational modifications (PTMs) are key mechanisms of epigenetics that modulate chromatin structures, termed as “histone code”. The PTMs on histone including acetylation, methylation, phosphorylation and novel acylations directly affect the accessibility of chromatin to transcription factors and other epigenetic regulators, altering genome stability, gene transcription, etc. Histone acetylation occurs primarily at multiple lysine residues on the amino-terminal of core histones, in response to various stimuli and plays vital roles in the regulation of gene expression, DNA damage repair, chromatin dynamics, etc. Mostly, histone H2A is primarily acetylated at Lys5, 9, 15, and 36; H2B is primarily acetylated at Lys5, 12, 15, 16, and 20. Histone H3 is primarily acetylated at Lys4, 9, 14, 18, 23, 27, 56, and 79. Histone H4 is primarily acetylated at Lys5, 8, 12, 16, and 20. Histone acetyltransferases (HATs) and histone deacetylases (HDACs) are major regulating factors.

Abbreviation

Histone H2B (Acetyl Lys46)

UniProt

P62807

Host

Rabbit

Reactivity

Human; Rat; Mouse

Immunogen

Acetylated human histone H2B (Lys46) peptide

Target

HIST1H2BG; H2B.1A; H2B/a; H2BFA; H2BFL; HIST1H2BC; H2BFH; HIST1H2BE; H2BFG; HIST1H2BF; H2BFK; HIST1H2BI; H2BC4; H2BC6; H2BC7; H2BC8; H2BC10

Clonality

Monoclonal, Recombinant

Clone

A101

Conjugation

Unconjugated

Applications

WB; ChIP; IF

Field of Research

Epigenetics and Nuclear Signaling

Purification

Protein A purified

Concentration

1 mg/mL

Dilution

6

Buffer

PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.

Shipping Conditions

Ice bag

Storage Conditions

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Calculated Molecular Weight

14 kDa

Observed Molecular Weight

15kDa

Isotype

IgG, κ