Recombinant Histone H4 (Acetyl Lys77) Monoclonal Antibody

Specifications

Catalog number

AN301408L-01

Size / count

50 µL

Dry ice

No

Hazardous

No

Discontinued

No

Background

Histone post-translational modifications (PTMs), known as the “histone code”, are key mechanisms of epigenetics that modulate chromatin structures. The PTMs on histone including acetylation, methylation, phosphorylation, and novel acylations directly affect the accessibility of chromatin to transcription factors and other epigenetic regulators, altering genome stability and gene transcription. Histone acetylation, tightly controlled by the opposing action of histone acetyltransferases (HATs) and histone deacetylases (HDACs), occurs primarily at lysine residues on the N-terminal tails of histones H2A (Lys5, 9, and 15), H2B (Lys5, 12, 15, 16, and 20), H3 (Lys4, 9, 14, 18, 23, 27, and 36), and H4 (Lys5, 8, 12, 16, and 20), and plays vital roles in the regulation of gene expression, DNA damage repair, and chromatin dynamics. Recent research has identified that H2BK120ac, H3.3K18ac, and H4K77ac are significantly associated with the survival of hepatocellular carcinoma (HCC) patients. Furthermore, H4K77ac has been linked to HCC recurrence. These findings suggest that H2BK120ac, H3.3K18ac, and H4K77ac may be potential prognostic factors for HCC.

Abbreviation

Histone H4 (Acetyl Lys77)

UniProt

P62805

Host

Rabbit

Reactivity

Human; Rat; Mouse

Immunogen

Acetylated human histone H4 (Lys77) peptide

Target

H4F; methyl histone H; methylated histone H; HIST2H; HIST1H; HISTH4H; histone H; HIST2H4B; H4/o; histone H4; HIST1H4A; FO108; H4/p; HIST1H4; Hist4h4; HISTH4H4; methyl histone H4; methylated histone H4; H4/A; H4FA; H4/I; H4FI; HIST1H4B; H4/G; H4FG; HIST1H4C; H4/B; H4FB; HIST1H4D; H4/J; H4FJ; HIST1H4E; H4/C; H4FC; HIST1H4F; H4/H; H4FH; HIST1H4H; H4/M; H4FM; HIST1H4I; H4/E; H4FE; H4/D; H4FD; HIST1H4K; H4/K; H4FK; HIST1H4L; H4/N; H4F2; H4FN; HIST2H4; HIST2H4A; H4; H4C1; H4C2; H4C3; H4C4; H4C5; H4C6; H4C8; H4C9; H4C11; H4C12; H4C13; H4C14; H4C15; H4-16; A; I; H4B; H4J; H4H; H4M; H4E; HIST1H4J; H4D; H4C; H4/p; histone H4; HIST1H4B H4/I

Clonality

Monoclonal, Recombinant

Clone

A103

Conjugation

Unconjugated

Applications

WB; IHC; IP; ChIP

Field of Research

Epigenetics and Nuclear Signaling

Purification

Protein A purified

Concentration

1 mg/mL

Dilution

6

Buffer

PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.

Shipping Conditions

Ice bag

Storage Conditions

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Calculated Molecular Weight

11 kDa

Observed Molecular Weight

11 kDa

Isotype

IgG, κ