Recombinant ANGPTL3 Monoclonal Antibody

Specifications

Catalog number

AN301438L-01

Size / count

50 µL

Dry ice

No

Hazardous

No

Discontinued

No

Background

Acts in part as a hepatokine that is involved in regulation of lipid and glucose metabolism. Proposed to play a role in the trafficking of energy substrates to either storage or oxidative tissues in response to food intake. Has a stimulatory effect on plasma triglycerides (TG), which is achieved by suppressing plasma TG clearance via inhibition of LPL activity. The inhibition of LPL activity appears to be an indirect mechanism involving recruitment of proprotein convertases PCSK6 and FURIN to LPL leading to cleavage and dissociation of LPL from the cell surface; the function does not require ANGPTL3 proteolytic cleavage but seems to be mediated by the N-terminal domain, and is not inhibited by GPIHBP1. Can inhibit endothelial lipase, causing increased plasma levels of high density lipoprotein (HDL) cholesterol and phospholipids. Can bind to adipocytes to activate lipolysis, releasing free fatty acids and glycerol. Suppresses LPL specifically in oxidative tissues which is required to route very low density lipoprotein (VLDL) -TG to white adipose tissue (WAT) for storage in response to food; the function may involve cooperation with circulating, liver-derived ANGPTL8 and ANGPTL4 expression in WAT.

Abbreviation

ANGPTL3

UniProt

Q9Y5C1

Host

Rabbit

Reactivity

Human; Mouse

Immunogen

Synthetic peptide derived from ANGPTL3 anti

Target

ANG; UNQ; PRO; FHBL; Angiopoietin; angiopoietin like; Angiopoietin-like protein; Angiopoietin-related protein; ANGPT; ANGPTL; ANGPTL3; ANG-5; ANGPT5; ANL3; FHBL2; angiopoietin like 3; Angiopoietin-5; Angiopoietin-like protein 3; Angiopoietin-related protein 3; ANL; UNQ153; PRO179; ANG-5; ANGPT5; ANL3; FHBL2

Clonality

Monoclonal, Recombinant

Clone

A133

Conjugation

Unconjugated

Applications

WB; IHC; IF

Field of Research

Cardiovascular; Signal Transduction; Metabolism

Purification

Protein A purified

Concentration

1 mg/mL

Dilution

WB 1:500-1:1000; IHC 1:50-1:100; IF 1:50

Buffer

PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.

Shipping Conditions

Ice bag

Storage Conditions

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Calculated Molecular Weight

54 kDa

Observed Molecular Weight

54 kDa

Isotype

IgG, κ