Recombinant Aurora B Monoclonal Antibody

Specifications

Catalog number

AN301441L-01

Size / count

50 µL

Dry ice

No

Hazardous

No

Discontinued

No

Background

Serine/threonine-protein kinase component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Involved in the bipolar attachment of spindle microtubules to kinetochores and is a key regulator for the onset of cytokinesis during mitosis. Required for central/midzone spindle assembly and cleavage furrow formation. Key component of the cytokinesis checkpoint, a process required to delay abscission to prevent both premature resolution of intercellular chromosome bridges and accumulation of DNA damage: phosphorylates CHMP4C, leading to retain abscission-competent VPS4 (VPS4A and/or VPS4B) at the midbody ring until abscission checkpoint signaling is terminated at late cytokinesis.

Abbreviation

Aurora B

UniProt

Q96GD4

Host

Rabbit

Reactivity

Human; Mouse

Immunogen

Recombinant human Aurora B fragment

Target

IPL; ARK; AIK; AIRK; PPP1R; STK; AIM; AIK2; AIM-1; AIM1; ARK2; AurB; IPL1; PPP1R48; STK12; STK5; aurkb-sv1; aurkb-sv2; AURKB; Aurora B; AIRK2; STK1

Clonality

Monoclonal, Recombinant

Clone

A136

Conjugation

Unconjugated

Applications

WB; IHC; IF

Field of Research

Cell Biology; Epigenetics and Nuclear Signaling; Signal Transduction; Cancer

Purification

Protein A purified

Concentration

1 mg/mL

Dilution

WB 1:500-1:1000; IHC 1:50-1:100; IF 1:50

Buffer

PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.

Shipping Conditions

Ice bag

Storage Conditions

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Calculated Molecular Weight

39 kDa

Observed Molecular Weight

39 kDa

Isotype

IgG, κ